Education:

• 2001, Degree in Biology, Department of Biology, University of Athens
• 2006, PhD in Biochemistry, Faculty of Medicine, University of Thessaly

Research Interests & Achievements:

• Investigation of cellular response to hypoxia and the human Hypoxia Inducible Factor 1 (HIF-1)
• Investigation of sumoylation of proteins in the cellular response to hypoxia.
• 2-year Post Doctoral fellowship from the Alexander von Humboldt foundation, Germany

Selected publications:

• A role for the Cajal-body-associated SUMO isopeptidase USPL1 in snRNA transcription mediated by RNA polymerase II. Hutten S, Chachami G, Winter U, Melchior F, Lamond AI. J Cell Sci. 2014, 127: 1065-78
• An association study between hypoxia inducible factor-1alpha (HIF-1α) polymorphisms and osteonecrosis. Chachami G, Kalousi A, Papatheodorou L, Lyberopoulou A, Nasikas V, Tanimoto K, Simos G, Malizos KN, Georgatsou E. PLoS One. 2013, 8:e79647.
• Oxygen-dependent secretion of a bioactive hepcidin-GFP chimera. Chachami G, Lyberopoulou A, Kalousi A, Paraskeva E, Pantopoulos K, Simos G. Biochem Biophys Res Commun. 2013, 435:540-5.
• Ubiquitin-specific protease-like 1 (USPL1) is a SUMO isopeptidase with essential, non-catalytic functions.Schulz S*, Chachami G*, Kozaczkiewicz L*, Winter U, Stankovic-Valentin N, Haas P, Hofmann K, Urlaub H, Ovaa H, Wittbrodt J, Meulmeester E, Melchior F.EMBO Rep. 2012, 13:930-8. * equally contributing first authors
• Transport of hypoxia-inducible factor HIF-1alpha into the nucleus involves importins 4 and 7.Chachami G, Paraskeva E, Mingot JM, Braliou GG, Görlich D, Simos G.Biochem Biophys Res Commun. 2009, 390:235-40. Atypical CRM1-dependent nuclear export signal mediates regulation of hypoxia-inducible factor-1alpha by MAPK.Mylonis I, Chachami G, Paraskeva E, Simos G.J Biol Chem. 2008 283:27620-7.
• Exposure of differentiated airway smooth muscle cells to serum stimulates both induction of hypoxia-inducible factor-1α and airway responsiveness to ACh.Chachami G, Hatziefthimiou A, Liakos P, Ioannou MG, Koukoulis GK, Bonanou S, Molyvdas PA, Simos G, Paraskeva E.Am J Physiol Lung Cell Mol Physiol. 2007, 293:L913-22.
• Identification of MAPK phosphorylation sites and their role in the localization and activity of hypoxia-inducible factor-1alpha.Mylonis I, Chachami G, Samiotaki M, Panayotou G, Paraskeva E, Kalousi A, Georgatsou E, Bonanou S, Simos G.J Biol Chem. 2006 281:33095-106.
• Bacterially produced human HIF-1alpha is competent for heterodimerization and specific DNA-binding.Chachami G, Paraskeva E, Georgatsou E, Bonanou S, Simos G.Biochem Biophys Res Commun. 2005, 331:464-70.
• Cobalt induces hypoxia-inducible factor-1alpha expression in airway smooth muscle cells by a reactive oxygen species- and PI3K-dependent mechanism.Chachami G, Simos G, Hatziefthimiou A, Bonanou S, Molyvdas PA, Paraskeva E.Am J Respir Cell Mol Biol. 2004, 31:544-51.